The effect of catalase on enzymatic saccharification of wheat straw slurry
[Examensarbete på avancerad nivå]
Enzyme inactivation during enzymatic saccharification is a problem because it causes a slowdown in saccharification before the substrate is completely converted into sugars. Lytic polysaccharide monooxygenases can, under certain conditions, produce hydrogen peroxide which causes release of reactive oxygen species. These reactive oxygen species can interact either directly or indirectly with enzymes, causing their inactivation. Another reason for enzyme inactivation is precipitation. It has been shown that up to 30% of proteins can precipitate during standard saccharification conditions. This project investigated the ef
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