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Evaluation of new methods to determine antimicrobial efficiency

Oceane Lancon
Göteborg : Chalmers tekniska högskola, 2015. 34 s.
[Examensarbete på avancerad nivå]

The work for this master's thesis, which was performed at Medibiome, was a part of a Vinnova-funded national project “Innovation mot Infektion” for the establishment and evaluation of a draft standard FprEN 16615 in order to evaluate different disinfection and analytical methods. Healthcare associated infections are a global issue and the need for new methods to reduce contamination and evaluate efficiency of disinfection is increasingly recognized in scientific, clinical and political communities. In order to evaluate the antibacterial efficiency of cleaning and disinfecting products, test floorings were inoculated with bacterial suspensions and an interfering substance, and wiped with a product. A unitary weight was used to standardize the wiping. The bacteria load on the surface was swabbed after wiping and the remaining bacterial load was evaluated after plate cultivation.The ATP bioluminescence analysis was used during a similar procedure to replace the plate cultivation method. The enzyme luciferase provided light emission while degrading the ATP contained into bacteria. The light emission is measured to determine the bacterial load on the surface. The compatibility of wiping materials and cleaning agents was also tested with a test suspension of S. aureus. The recovery of S.aureus and P. aeruginosa after different drying times on surfaces was studied. The results show that three disinfectants meet the requirements of the standard FprEN 1661: DES45, Rely+On™ Virkon® and Wet Wipe. Input was transmitted to the standard committee, especially regarding the need to sterilize the wipes, to standardize and improve the release of bacteria from the swab, and to decrease the weight of the granite block for more clinical relevance. ATP analysis could be performed without background by using an interfering substance modified with Triton X-100 at 0.2%. It seems to be a promising technique, as it is both fast and cost-efficient but further study needs to be done in order to solve technical issues and standardize the method

Nyckelord: Healthcare associated infection, disinfection, cleaning, wiping, bacteria, ATP

Publikationen registrerades 2015-04-14. Den ändrades senast 2015-04-14

CPL ID: 215190

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